Coding

Part:BBa_K3778023:Design

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-09-05)


Thrombin releasable D2A21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We modify the last two amino acids, glycine(G) and serine(S), in the thrombin cleavage site to fit the first two amino acids of D2A21 (BBa_K3778007) to avoid extra residues at the N-terminal of D2A21 after thrombin cleavage.



Source

D2A21 is cloned from Cecropin and this part is modified by CCU_Taiwan team.


References